| Caspase9

Caspase-9 is an enzyme that is encoded by the CASP9 gene. It is an initiator caspase,critical to the apoptotic pathway found in many tissues.Caspase-9 homologs have been identified in all mammals for which they are known to exist, such as Mus musculus and Pan troglodytes.Caspase-9 belongs to a family of caspases, cysteine-aspartic proteases involved in apoptosis and cytokine signalling.Apoptotic signals cause the release of cytochrome c from mitochondria and activation of apaf-1 (apoptosome), which then cleaves the pro-enzyme of caspase-9 into the active dimer form.Regulation of this enzyme occurs through phosphorylation by an allosteric inhibitor, inhibiting dimerization and inducing a conformational change.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.61	2.12	1.36	0.79	0.44	0.27	0.21	0.09
Corrected OD	2.52	2.03	1.27	0.7	0.35	0.18	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human Caspase9 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	85-101	97
Cell Culture Supernates(n=8)	96-109	105

 

| Linearity

Samples were spiked with high concentrations of Human Caspase9 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-96	90-110
	Average (%)	92	96
1 : 4	Range (%)	89-103	105-115
	Average (%)	94	108